摘要
目的:建立并比较野生和栽培品种防风中升麻苷、5-O-甲基维斯阿米醇苷HPLC测定方法和HPLC指纹图谱。方法:含量测定采用岛津C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-水(40∶60),检测波长254 nm,流速1.0 mL.min-1,柱温30℃。HPLC指纹图谱测定采用资生堂MGⅡC18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-水,梯度洗脱,检测波长254 nm,流速1.0 mL.min-1,柱温30℃。结果:升麻苷加样回收率为99.6%,RSD 0.72%(n=6);5-O-甲基维斯阿米醇苷加样回收率为102.6%,RSD 0.88%。野生与栽培品种升麻苷质量分数分别为(4.96±2.59),(3.61±1.82)mg.g-1;5-O-甲基维斯阿米醇苷质量分数分别为(3.91±2.09),(4.37±2.02)mg.g-1。野生与栽培品种升麻苷和5-O-甲基维斯阿米醇苷质量分数无显著性差异。在此条件下所建立的指纹图谱中防风各成分得到有效分离。结论:升麻苷、5-O-甲基维斯阿米醇苷HPLC测定方法简便,结果准确,HPLC指纹图谱分析可作为防风药材质量控制及分类评价的依据。
Objective： To develop HPLC methods for the determination of prim-O-glucosylcimifugin and 5-O-methylvisammisoide in Saposhnicovia divaricata and of HPLC fingerprint to compare the wild and culture varieties.Method： Conditions of determination： Shimadzu C18 column（4.6 mm×150 mm,5 μm）,methanol-water（40∶60） as mobile phase with the flow rate of 1 mL·min-1.The detection wavelength was 254 nm.Conditions of HPLC fingerprint： MG Ⅱ C18 column（4.6 mm ×250 mm,5 μm）,the mobile phase was acetonitrile-water with the flow rate of 1 mL·min-1,using linear gradient elution,the column temperature was 30 ℃.Result： The average recovery of prim-O-glucosylcimifugin was 99.6%（RSD 0.72%,n=6）.The average recovery of 5-O-methylvisammisoide was 102.6%（RSD 0.88%,n=6）.The contents of prim-o-glucosylcimifugin in wild and culture varieties were（4.96±2.59） and（3.61±1.82） mg·g-1 respectively.The contents of 5-O-methylvisammisoide were（3.91±2.09） and（4.37±2.02） mg·g-1 respectively.The compositions of S.divaricata were effective separated under the conditions of HPLC fingerprint.Conclusion： The HPLC determination method of prim-O-glucosylcimifugin and 5-O-methylvisammisoide is convenient and accurate.The HPLC fingerprint analysis method could be a basis for quality control and classification evaluate of S.divaricata.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2010年第13期1731-1734,共4页
China Journal of Chinese Materia Medica
基金
科研院所社会公益研究专项(2005DIA1J172)
关键词
防风
升麻苷
5-O-甲基维斯阿米醇苷
指纹图谱
Saposhnicovia divaricata
prim-O-glucosylcimifugin
5-O-methylvisammisoide
fingerprint
