摘要
目的设计新型结构磁性脂质体,并通过自组装法制备。方法采用共沉淀法制备Fe3O4磁性纳米粒子,采用薄膜分散法制备多柔比星脂质体,测定Fe3O4粒子和多柔比星脂质体的Zeta电位,根据电荷性质选择适当的聚电解质,依次将聚电解质和Fe3O4粒子组装到多柔比星脂质体表面,得到磁性脂质体,并采用透射电镜(TEM)观察其形貌。结果 Fe3O4粒子和多柔比星脂质体的Zeta电位分别为-22.0、-22.6 mV,自组装过程中选择阳离子聚电解质PDADMAC作为中间层,磁性脂质体的TEM照片显示,其具有较规则的球形轮廓,表面均匀吸附有大量的Fe3O4磁性粒子使得脂质体表面略显粗糙,证实了新型结构磁性脂质体的成功构建。结论采用静电自组装法可以制备新型结构磁性脂质体。
OBJECTIVE To design and prepare new-structured magnetic liposome by self-assembly method. METHODS Fe3O4 magnetic particles were prepared by co-precipitation. Doxorubicin liposomes were prepared by film dispersing method. The Zeta potential of the Fe3O4 magnetic particles and doxorubicin liposome was detected, and then appropriate polyelectrolyte interlayer was selected. Magnetic liposome was obtained by alternately assembling polyeleetrolyte and Fe3O4 magnetic particles onto doxorubicin liposome. The morphology of the magnetic liposome was characterized by transmission electron microscopy （TEM）. RESULTS The Zeta potentials of the Fe3O4 magnetie particles and doxorubicin liposome were -22. 0 and -22. 6 mV, respectively, and the cation polyelectrolyte （PDAD- MAC） was selected as interlayer. TEM photograph of the magnetic liposome indicated that the liposome showed regular spherical profile and had rough surface for the uniform adsorption of Fe3O4 particles, which provided direct evidence for successful fabrication of the newstructured magnetic liposome. CONCLUSION The new-structured magnetic liposome can be prepared by self-assembly method.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2010年第12期919-922,共4页
Chinese Pharmaceutical Journal
基金
陕西省自然科学基金资助项目(2009JQ2013)
西北工业大学科技创新基金(2008KJ02041)
关键词
磁性
脂质体
新型结构
自组装
magnetic
liposome
new-structured
self-assembly
