摘要
以催醒胺为内标．建立TRP－HPLC法规定人血浆中富马酸比索洛尔的浓度。色谱柱采用Nova－pakC18分析柱，流动相为己腈-甲醇-磷酸（0．09mol／L）-水（34：10：6：50v／v），荧光检测波长232／300nm（Ex／Em）。血浆样品经碱化后乙酸提取可获得良好分离，血药浓度在4～128ng／ml范围内呈线性关系，相关系数0．9998，最低检测浓度为2ng／ml。生物样品的平均方法回收率98．34％～105．43％，平均提取回收率75．01％～97．48％，日内日间精密度RSD0．73％～9．06％。
A method using fluorimetric detection was developed for bisoprolol hemifurnarate concertration in plasma by RP-HPLC, The analytical column was Nova-pak C18 (4.0mm ×15cm, 4 μm). The mobile phase consisted of a mixture of acetonitrile-methanol-0.09 mol/L phosphoric acid-water(34: 10: 6:50). The detective wavelength was 232/300nm(Ex/Em). The bisoprolol in plasma can be well purified after extraction at alkaline with diethyl ether. The calibration curve was linear in the range from 4 to 128ng/ml (r = 0.9998), the detection limit of this method was 2ng/ml. The method recoveries in samples were 98. 34 % ~ 105.43 %,the extraction recoveries were 75.01 % ~ 97. 48 %,and the RSD of within-day and dayto-day were 0. 73 % ~ 9.06 %.
出处
《海峡药学》
1999年第1期39-41,共3页
Strait Pharmaceutical Journal
