摘要
根据绿竹纤维素合成酶(cellulose synthase)基因的保守区序列设计引物,以雷竹cDNA为模板,采用PCR方法,成功扩增出1个含有完整阅读框架的cDNA序列,长度为3 385 bp,共编码1 056个氨基酸,将其命名为PpCesA1基因。氨基酸序列的分析结果表明,PpCesA1与其他纤维PeCesA4素合成酶有较高的同源性,同绿竹序列相似性高达94%,且其序列具有典型的Cellulose_synt-GT-A结构域,推测此PpCesA1为雷竹纤维素合成酶基因。对雷竹不同组织采用半定量方法研究该基因的表达情况,结果表明该基因在不同组织中的表达有明显差异。
A cellulose synthasese protein gene from P.praecox.C.d.Chu et C.S.Chao named as PpCesA1,was cloned through PCR using primers designed according to the cellulose synthasese protein genes conserved region of Bambusa multiplex.The coding region of the genomic clone of PpCesA1 was continuous.The cDNA of PpCesA1 contained an open reading frame of 3 385 bp and codes for a protein of 1 056 aa.The database search using the amino acid sequence as query showed high homology to several cellulose synthasese proteins,especially the PpCesA1 sequence showed the maximum homology（94% identity） to Bambusa multiplex（subspecies cellulose synthasese protein gene）,and it had a typically conserved domain of Cellulose_synt-GT-A.Therefore it could be predicted that the PpCesA1 could be an cellulose synthasese gene in bamboo.The result of semiquantitative RT-PCR in differet tissues of P.praecox.C.d.Chu et C.S.Chao showed that the expression of PpCesA1 gene was different.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2010年第3期535-540,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
浙江省科技厅项目(2451001049)
关键词
雷竹
纤维素合成酶
克隆
表达分析
P.praecox.C.d.Chu et C.S.Chao
cellulose synthase
cloning
expression analysis
