摘要
MtN3/saliva基因家族是含有2个MtN3/saliva跨膜结构域的膜整合蛋白,其成员广泛存在于真核生物中.目前对该家族功能研究较少,对其跨膜结构域的分子功能还不清楚.在拟南芥中该家族有18个成员,除了最近克隆的RPG1以外其他MtN3/saliva基因的功能均不清楚.对拟南芥MtN3/saliva基因家族进行了较为全面的分析,包括基因结构、染色体分布、蛋白结构域、系统进化关系、基因表达谱等.对其中预测在花药中高表达的4个基因进行了实时定量PCR分析,证实有3个基因在花中高效表达.这些结果促进了对MtN3/saliva基因家族的深入了解.
MtN3/saliva protein family genes are found universally in eukaryotes, and the majority of these proteins usually contain two copies of MtN3/saliva domain. Very few of the MtN3/saliva proteins have been characterized. And the two copies of MtN3/saliva domain＇ s function are unclear. In this study, eighteen genes of the MtN3/saliva family were identified in Arabidopsis thaliarm, while most of them were functionally unknown, except RPG1 cloned recently. The MtN3/saliva family in Arabidopsis was extensively analyzed,including gene structure, gene localization in genome, protein motif organization, phylogenetic relationship of family members and gene expression pattern. According to the expression data from the e-FP Browser and Genevestigater websitc, four MtN3/saliva family genes were highly expressed in anther and/or mature pollen grains, and their expression have been further verified by Real-time RT-PCR in our research. These results facilitate us to further understand the function of MtN3/saliva family in plant.
出处
《上海师范大学学报（自然科学版）》
2010年第3期290-298,共9页
Journal of Shanghai Normal University(Natural Sciences)
基金
国家自然科学基金(30770206)
