摘要
目的寻求一种简单、快速测定尿中无机砷的方法。方法用碳酸铵分离尿中的无机砷。于２５ｍｌ尿样中加入１５ｍｌ１％碳酸铵溶液，２４０次／ｍｉｎ振摇１ｈ；加入１ｍｌ浓硫酸，８０℃水浴１０ｍｉｎ；冷却后过滤，用ＡｇＤＤＣ法测定滤液中的无机砷。结果回收率为９７．６８％～１１０．８６％；重复试验的标准差和变异系数为０．０２７２μｇ和３．６６％。用此法测３８名健康人尿中的无机砷，结果为７．９９×１０－３±４．１９×１０－３ｍｇ／Ｌ，无机砷占尿总砷的百分比为５．１２％～２８．３０％，Ｐ５０为１５．３８％。
Objective To find out a rapid and simple method to determine the amounts of inorganic arsenic (iAs) in urine.Methods Ammonium Carbonate [(NH 4) 2CO 3] was used to seperate iAs from urine.After the addition of 15 ml of 1% (NH 4) 2CO 3 solution into 25 ml of urine sample,the sample was stirred at 240 times per minute for an hour.After the addition of 1 ml of strong sulphuric acid,the sample was incubated in water at 80℃ for 10 minutes.Filtering the solution after it cool down,iAs in filtrate was determinded by AgDDC assay.Results The recovery rates were from 97.68% to 110.86%;the standard deviation and CV were 0.0272μg and 3.36%,respectively.38 urine samples from a normal group were also analyzed.The amount of iAs was 0.00799±0.00419mg/L;the rate of iAs to total As was over the range of 5.12% to 28.30%;P 50 is 15.38%.Conclusion iAs in urine can be measured by this method.
出处
《中国地方病学杂志》
CAS
CSCD
1999年第1期65-66,共2页
Chinese Jouranl of Endemiology
基金
国家自然科学基金
关键词
无机砷
碳酸铵
尿
砷化氰
测定
Inorganic arsenic (iAs) Ammonium Carbonate Urine
