摘要
目的观察小剂量阿斯匹林对同种异基因移植受体大鼠慢性移植肾肾病的作用并探讨可能的作用机制。方法建立大鼠肾移植慢性排斥反应模型,移植受体大鼠随机分为2组(n=10),均自术前1d开始每日以环孢素A5mg/kg灌胃,治疗组每日给予阿斯匹林5mg/kg灌胃,对照组每日给予生理盐水2ml灌胃,术后8周采静脉血测定血清肌酐、尿素氮水平;处死动物取移植肾组织进行H-E染色及免疫组化染色(测定TGF-β1表达)。结果阿斯匹林治疗组大鼠血肌酐、尿素氮升高程度,组织病理损害及TGF-β1表达程度均较生理盐水对照组轻(P<0.05)。结论肾移植术后在常规抗排斥治疗基础上使用小剂量阿斯匹林对慢性移植肾肾病有治疗作用,其机制可能与致纤维化因子TGF-β1的表达减少有关。
Objective To observe the effect of low-dose aspirin on allogeneic chronic allograft nephropathy in renal recipient rats and the possible mechanisms.Methods Chronic allograft nephropathy model of rats were established and the recipient rats were randomly divided into 2 groups with 10 in each group.Cyclosporine A （5 mg/kg） was administered as a basic treatment one day before surgery to prevent and treat acute rejection.Aspirin therapy group was given oral aspirin （5 mg/kg） daily,and control group were given oral saline 2 ml/d.Animals were sacrificed 8 weeks after operation and the serum creatinine,urea nitrogen levels were determined.Renal histomorphology and immunohistochemistry approaches were used to examine TGF-β1 expression.Results The severity of pathological lesion,increase of serum creatinine and blood urea nitrogen levels,and expression of fibrosis associated factor TGF-β1 in aspirin therapy group were significantly slighter than those of the saline control group（P0.05）.Conclusion Low-dose aspirin in addition to routine anti-rejection treatment can be used for treatment of chronic allograft nephropathy in rats,which might be associated with the decreased expression of anticoagulation factor TGF-β1.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2010年第6期650-652,共3页
Academic Journal of Second Military Medical University
关键词
肾移植
阿司匹林
移植物排斥
转化生长因子Β1
renal transplantation
aspirin
graft rejection
transforming growth factor beta 1
