摘要
目的探讨抗Ｆａｓ单抗、Ｂｃｌ２反义脱氧寡核苷酸在诱导小儿急性淋巴细胞白血病（简称急淋）细胞凋亡中的作用。方法小儿急淋白血病细胞分别与Ｂｃｌ２反义脱氧寡核苷酸、抗Ｆａｓ单抗培养，或与两者一起培养，再分别定量检测其凋亡细胞。结果Ｂｃｌ２反义脱氧寡核苷酸或抗Ｆａｓ单抗分别与１７例及８例小儿急淋白血病细胞培养４８小时后，能各自诱导（３５．７±９．４）％及（３９．４±８．２）％细胞凋亡。当它们共同作用于８例小儿急淋白血病细胞时可诱导（６９．８±１３．１）％白血病细胞凋亡。结论Ｂｃｌ２反义脱氧寡核苷酸与抗Ｆａｓ单抗均能诱导小儿急淋白血病细胞的凋亡，二者联合应用可显著加强其诱导凋亡的作用。
Objective To explore the function of anti Fas monoclonal antibody (MoAb) and Bcl 2 antisense oligodeoxynucleotides (ASON) in mediating apoptosis of childhood acute lymphocytic leukemia (ALL)cells.Methods The percentage of apoptotic cells was detected after childhood ALL cells were incubated with Bcl 2 ASON, anti Fas MoAb or with both of them, respectively.Results All cells were incubated with Bcl 2 ASON (17 samples) or anti Fas MoAb (8 samples) for 48 hours, and the percentage of apoptotic leukemic cells reached to (35.7±9.4)% and (39.4±8.2)% respectively. The ratio of apoptotic cells from the 8 ALL samples increased to (69.8±13.1)% when they were incubated with both Bcl 2 ASON and anti Fas MoAb.Conclusion The use of both Bcl 2 ASON and anti Fas MoAb may probably provide an effective method for clinical treatment of childhood acute lymphocytic leukemia.
出处
《中华儿科杂志》
CSCD
北大核心
1999年第1期21-23,共3页
Chinese Journal of Pediatrics
基金
国家自然科学基金
