摘要
本工作在单发佐剂性关节炎慢性痛模型大鼠上比较观察了不同时间间隔和不同强度的多次１００Ｈｚ经皮神经电刺激（ＴＥＮＳ）的治疗效果，并对炎症和ＴＥＮＳ过程中Ｐ物质（ＳＰ）和强啡肽（Ｄｙｎ）的变化规律及其作用进行了初步探讨。结果表明：（１）用小量的油包水型完全弗氏佐剂（ＣＦＡ）进行大鼠单侧踝关节腔内注射可造成单发性关节炎慢性痛模型。（２）刺激间隔时间和刺激强度均是影响多次ＴＥＮＳ治疗慢性炎症痛的重要因素。治疗的适宜间隔时间：急性期（１～３周），每周２次最优；稳定期（４～９周），每周１次较好。刺激强度：弱刺激的疗效较好。（３）多次较弱ＴＥＮＳ治疗慢性炎症痛的过程中脊髓ＳＰｉｒ基础释放量较致炎对照组减少，这可能是多次ＴＥＮＳ治疗慢性炎症痛的机制之一。（４）慢性单发性关节炎１周时，脊髓ＤｙｎＡ（１１７）ｉｒ的基础释放量升高，３周后持续低于正常水平。关节炎１周和９周时，脊髓鞘内注射ＮｏｒＢＮＩ均可剂量依赖性地引起炎症关节的痛敏加重，提示脊髓强啡肽κ受体系统在慢性炎症痛中发挥着对伤害性信息的紧张性抑制作用。
Aims: To establish a reliable animal model of chronic arthritic inflammatory pain, to find the optimal parameters and appropriate intervals of repeated 100 Hz transcutaneous electrical nerve stimulation (TENS) for the treatment of the chronic arthritic pain , and to explore the possible central neurochemical mechanisms. The results: (1) Injection of water in oil type complete Freund's adjuvant into the tibio tarsal joint of the rat produced a reliable, reproducible monoarthritic model. (2) The interval between 2 sessions of stimulations and the intensity of stimulation were important factors determining the therapeutic effects of repeated 100 Hz TENS on both acute and stable period of monoarthritis. (a) For acute period of monoarthritis, the optimal frequency is twice a week, while in the stable period, once a week seems better. (b) The effects of TENS in three intensities: the weaker stimulation was found to produce better results. (3) In the period from 2 to 9 weeks, the levels of spinal release of SP ir in the TENS group were lower than the control group. This may constitute one of the possible neuro chemical mechanisms underlying the therapeutic effects of multiple TENS treatment. (4) In both acute and stable period of adjuvant induced monoarthritis, intrathecal microinjection of Nor BNI produced dose dependent increase of arthritic flexion pain scores (i.e. hyperalgesia), suggesting that spinal dynorphin/kappa system plays a role in suppressing arthritic pain.
出处
《生理科学进展》
CAS
CSCD
北大核心
1999年第1期35-37,共3页
Progress in Physiological Sciences
基金
中国国家科委脑功能研究攀登计划
美国国立卫生研究院科研基金
关键词
关节炎
经皮电神经刺激
强啡肽
Adjuvant
Monoarthritis
Transcutaneous electrical nerve stimulation (TENS)
Substance P
Dynorphin
Nor BNI
