摘要
应用免疫组织化学和邻位切片法，研究了猫基底前脑胆碱能皮质投射神经元区的Ｐ物质样免疫反应神经元的分布特征，及其与胆碱乙酰化酶样免疫反应神经元的分布关系。从内侧隔核至Ｍｅｙｎｅｒｔ基底核，２种神经元分布范围相近，且形态、大小相似。但在邻位切片，未见分别呈此二免疫反应的同一神经元的对应剖面。Ｐ物质样免疫反应神经元在内侧隔核和斜角带核数量较多，但在基底核明显减少，在Ｃｈ４间质部及腹侧苍白球连合下部仅为偶见。结果表明，在内侧隔核－斜角带核－基底核，Ｐ物质能神经元主要分布在内侧隔核－斜角带核，多数Ｐ物质能和胆碱能神经元各自归属不同的神经元亚群。以上提示，在隔－海马投射系中，除胆碱能和ＧＡＢＡ能神经元，Ｐ物质能神经元是又一重要成分，可能对新皮质－海马－新皮质记忆环路有重要影响。
The distribution of the substance P like immunoreactive (SP LI) neurons in the basal forebrain cholinergic corticopetal neuron areas in the cat was studied and compared with that of the choline acetyltransferase like immunoreactive (ChAT LI) neurons in the paired consecutive sections of the corresponding areas.A continuum of ChAT LI neurons were distributed in the medial septal nucleus (MS),nuclei of diagonal band,vertical and horizontal divisions (NDBV/H),nucleus basalis of Meynert (NBM),subcommissural ventral pallidum (sVP),and the areas of interstitial elements of Ch4,e.g.the internal capsule (ic) and nucleus of anterior commissure (Nac).The distribution of the SP LI neurons closely resembled that of the ChAT LI neurons with similar shape and size in the corresponding areas of the MS NDB NBM continuum.However,no perikarya in the paired surfaces of two adjacent sections could be really identified as bisected neurons being SP and ChAT LI respectively.The number of the SP LI neurons,compared with that of the ChAT LI neurons was more in MS,almost the same in NDBV/H,much less in NBM,and only a few in the interstitial elements of Ch4 areas and sVP.The results demonstrated that the majority of the SP LI neurons in the MS NDB NBM were mainly contained in the MS NDB and most of the SP LI neurons in the cholinergic neuron areas belonged to a subdivision with different chemical property.We suggested that the SPergic neurons in the MS NDB might be one of the main components,in addition to the cholinergic and GABAergic neurons,in the septohippocampal projection system and might play an important role in modulation of the memory functioning neocortex hippocampus neocortex circuit.
关键词
P物质
胆碱乙酰化酶
斜角带核
神经元
Substance P,Choline acetyltransferase,Septal area,Diagonal band nuclei,Nucleus basalis of Meynert
