摘要
用（ＳＤＳ）ＰＡＧＥ对外源豌豆ＤＮＡ导入小麦体的不良变异后代种子蛋白质和酯酶同工酶（ＥＳＴ）、超氧化物歧化酶（ＳＯＤ）、过氧化同工酶（ＰＯＤ）进行分析，发现变异小麦的种子蛋白质消减４个组分带（９２ｋＤ、８２ｋＤ、６４ｋＤ、５８ｋＤ），ＥＳＴ和ＰＯＤ消减２条酶谱带，ＳＯＤ的活性明显降低，并且变异小麦植株的发育生长表型呈现出脆弱、早衰迹象，表明导入外源ＤＮＡ抑制了某些基因的表达。
Analysing seed protein,esterase isozyme (EST),superoxide dismutase (SOD),peroxidase isozyme (POD) of variant wheat derived from introducing exogenous pea DNA into common wheat via (SDS) PAGE showed that the seed protein decreased 4 components,EST and POD reduced 2 isozyme bands,SOD activity was also striking lower,and the growth developmental phenotype of the wheat plants appeared weak,early wane phenomena.These variations can stably be expressed in successive progenies without separation.This indicated that introducing exogenous DNA suppress or affect the level of gene expression.Meanwhile the related factors or causes resulting from the suppression are also discussed.
出处
《西北植物学报》
CAS
CSCD
1999年第1期41-45,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省科学基金
