摘要
桃果实不耐贮运，特别是溶质水蜜桃、珐I大11＿程技术的发展使番茄果实实现延熟保鲜'－'、人f［］也试图通过乙烯合成的关键酶的］i义HNA技术来延长桃果实的贮运期。：ICC氧化酶是乙烯生物合成的关键酶之一，ACC氧化酶4peJL从番茄'、矮牵牛。。。桃卜'、苹果、'等许多植物...
RNA-based PCR amplification and Southem blotting of ACC oxidase cDNAs from peach (Prunuspersica L. ) fruits indiceated that ACC oxidase mRNA was detected when fruit began to soften at about 75 d afterbloom, and its accumulation peaked when the fruit mostly softened (about 1 kg/cm2). ACC oxidase mRNAwas undetectable in green fruits and unripe fruits, but wounding and ethylene could induce ACC oxidase expression of these fruits. Similarly, ACC oxidase mRNA was undetectable in the alabastrum and petal just afteranthesis and rapidly accumulated during sellescence, and was indueed by ethylene. Petal was more sensitive toethylene than fruit. An increase in ACC oxidase mRNA level of leaf was found after 0. 5 to 8 h of wounding,but ACC oxidase mRNA accumulated more rapidly after wounding in leaf than in fruit. Ethylene seemed tohave little effect on ACC oxidant expression of leaf.
基金
浙江省自然科学基金
农业部"九五"重点课题
