摘要
红莲（NelumbonuciferaGaertn）成熟于叶总蛋白含量达24．35g／100g干样品，其贮存蛋白（SP）的积累模式与豆科种子相似，即随成熟度的提高，SP猛增至占总蛋白含量的86％以上。对莲子不同发育阶段子叶蛋白SDS-PAGE图谱的光密度测定表明：莲子叶蛋白有12个主要SP亚基（SP1－12）。按分子量（MW）大小和积累顺序可分3组：A组是98kD和93kD的2个亚基，MW最大，积累最晚，但量最多；B组是3个亚基，MW为55－50kD，大小居中，积累最早，量最少；C组的7个亚基MW最小，在27－14kD之间，积累较早，量较多。对莲的不同品种、不同器官进行分析后发现，它们都有1个主峰为68kD的多连峰的代谢蛋白亚基，可能是莲共有的蛋白亚基。
The content of total proteins (TP) in the mature cotyledon of red lotus (Nelumbo nucifera Gaertn)reached 24. 35g/100 g dry sample: the accumulation pattern of its stoop proteins (SP) was similar to that ofLeguminosae seeds, i. e. with increasing maturity, the content of SP reached abruptly to more than 86% of theTP content. Result from optical densitometry of protein SDS-PAGE in different developmental stages of the lotus cotyledon indicated that the lotus cotyledon SP included 12 major subunits (SP 1-12). In respect of MWand accumulation time, they might be divided into 3 pops. Group A had 2 subunits with the highest MW (98kD and 93 kD) and the latest accumulation time that had the highest SP content. Group B had 3 subunits withmoderate MW of 55-50 kD, their earliest accumulation time, and least SP content. And group C included 7subunits with the lowest MW of 27-14 kD, moderately early accumulation time, and high SP comparativelycontent. By comparing the results of SDS-PAGE analysis among different varieties and organs of lotus, therewas a common metabolic protein subunit with multiply connected peak. ItS MW was 68 kD, which might be acommon subunit of the lotus species.
基金
国家自然科学基金!39570047
关键词
莲
子叶
贮存蛋白
亚基
积累模式
Nelumbo nucifera, Cotyledon, Major subunits of storage proteins, Accumulation Pattern
