摘要
用含胶原酶、弹性蛋白酶的无钙溶液和改良的回复式液体运动来酶解和机械分离兔心窦房结自律细胞。为防止“衰减”现象的出现（ｒｕｎｄｏｗｎ），运用含ＡｍｐｈｏｔｅｒｉｃｉｎＢ的电极液作穿孔膜片箝。通过穿孔膜片箝的形成过程，记录分离细胞的自律性动作电位、慢内向离子流、外向钾流和起搏离子流Ｉｆ。
Sinus node cells were isolated from rabbit heart by both enzymatic digestion in Ca free medium containing collagenase and elastase and an improved mechanical trituration method. In order to prevent the “run down” phenomena, amphotericin B in pipette solution was used to make perforated patch , the process of which was described in details. The spontaneous action potential, slow Ca in ward current , outward K current and pacemaker current (I f) from the isolated SN cells were recorded with the methods,which demonstrated that the improved methods are dependable and effective.
出处
《中国应用生理学杂志》
CAS
CSCD
1999年第1期89-92,共4页
Chinese Journal of Applied Physiology
基金
国家自然科学基金
上海高校发展基金
上海第二医科大学重点基金
关键词
窦房结
穿孔膜片箝
游离
跨膜离子流
sinus node
perforated patch
isolation
transmemborane current
