摘要
利用四唑盐(MTT)比色法检测观察菊科植物中分离出的三种倍半萜类化合物,异土木香内酯(1),1氧--乙酰大花旋覆花内酯(2)和大花旋覆花内酯(3)对人脑神经胶质瘤细胞、肝癌细胞、人黑色素瘤和人乳腺癌脑转移肿瘤细胞生长的影响,探讨这三种倍半萜类化合物的体外抗肿瘤细胞增殖作用以及化合物结构与活性之间的构效关系。结果发现异土木香内酯对人脑神经胶质瘤细胞、人肝癌细胞、人和色素瘤细胞和人乳腺癌脑转移肿瘤细胞的增殖均有明显的抑制作用,1氧--乙酰大花旋覆花内酯和大花旋覆花内酯即使在100μmol/L的高浓度下,对四种肿瘤细胞的增殖不显示抑制活性,推测A环-开环可能是导致桉叶烷型倍半萜内酯的体外抗肿瘤细胞增殖作用消失或减弱的原因。
Three sesquiterpene lactones isoalantolactone （1）, 1-O-acetylbritannilac tone （2） and britannilactone （3） were isolated from ＇the roots of Inula hdenium and the flowers of Inula japonica. This paper useed MTT assay to evaluate the antitumor activities of three sesquiterpenoids to the cell line of U251SP, HLE, MM1-CB and KT, and to explore the relationship between structure and activity. MTT assay showed that isoalantolactone （ 1 ） exhibited excellent anti-growth activities to U251SP, HLE, MM1 -CB and KT, while 1 -O-acetylbritannilac tone （2） and britarmilactone （3） demonstrate weak or moderate anti-growth activities to U251SP, HLE, MM1-CB and KT cell lines even in the concentration of 100 μmol/L. This result indicated that anti-growth activities to four cell lines have closely relationship with the structures, 1, 10-seco of eudesmane sesquiterpene lactone lead to the lossess or decrease of anti-growth activity.
出处
《天然产物研究与开发》
CAS
CSCD
2010年第3期506-509,共4页
Natural Product Research and Development
基金
河北省引进留学人员基金课题(2008-2010)
河北省自然科学基金课题(08B032)
河北省中医药管理局科研课题(2006053)
关键词
菊科
土木香
旋覆花
倍半萜类化合物
肿瘤细胞
增殖活性
Inula helenium
Inula japonica
sesquiterpenes compounds
human tumor cell lines
anti-growth activity
