摘要
用８种方法保藏１８个Ｂｔ菌株５～２３年，结果表明，不同保藏方法对Ｂｔ菌株的存活率和毒效存在不同程度的影响。这些菌株平均存活率为２９．３％～８０．３％。除保藏５年的ＭＰ┐３４２外，较高者有７９００７（１８年）５７．５％，８２６１２（１５年）５６．９％；最低为０１６（２２年）２９．３％。不同保藏方法平均存活率排序：土壤保藏Ａ（５５．５％）＞沙管保藏Ａ（５３．０％）＞虫尸保藏Ａ（４７．５％）＞土壤保藏Ｂ（４３．５％）＞沙管保藏Ｂ（３８．７％）＞虫尸保藏Ｂ（３６．６％）＞石蜡保藏（３１．６％）＞滤纸保藏（３０．１％）。沙管。
methods for preservation of Bt cultures were tested through a period of 5-23 years The 18 Bt strains showed different survival rates, averaging 29 3%-80 3% after storage The survival rates for strain 79007 was 57 5% (18 years), for strain 82612 was 56 9% (15 years), for strain 016 was 29 3% (22 years) The average survival rates for different storage methods were soil tube A method (55 5%), sand tube A method (53 0%), insect corpse A method (47 5%), soil tube B method (43 5%), sand tube B method (38 7%), insect corpse B method (36 6%), paraffin wax tube method (31 6%) and filter paper tube method (30 1%) The sand tube, soil tube and insect corpse tube methods could be used for the preservation of Bt culture
出处
《中国生物防治》
CSCD
1999年第1期19-22,共4页
Chinese Journal of Biological Control
基金
国家"七五"
"八五"攻关项目
关键词
苏云金杆菌
存活率
毒效
菌种保藏
acillus thuringiensis survival rate virulence culture conservation
