摘要
为了提高假丝酵母发酵过程中的产量,本实验进行了发酵研究,确定了最佳发酵装液量为50mL,接种量为10%。并在装液量为50mL,接种量为10%的250mL锥形瓶中,进行了4组蔗糖添加方案:a·每1h添加0·25g蔗糖(流加液0·25mL);b·每2h添加0·5g蔗糖(流加液0·5mL);c·每3h添加0·75g蔗糖(流加液0·75mL);d·每4h添加1g蔗糖(流加液1mL)。第一次添加蔗糖后,隔4h再添加蔗糖,添加24h后,培养基蔗糖总浓度达到100g/L,停止添加,继续培养48h,测干重,结果方案a得到的假丝酵母产量最高。
This experiment determined the best liquid volume was 50mL and the best of inoculum was 10%,in a high concentration of sucrose（100g/L sucrose concentration）of the Candida Berkhout culture fermentation. And taking 4 kinds of add sugar program in the conical 250mL bottle with volume liquid was 50mL,inoculum was 10% a.add 0.25g sucrose per 1h（liquid fed 0.25mL）,b.add 0.5g sucrose each 2h（liquid fed 0.5mL）,c.add 0.75g sucrose per 3h（liquid fed 0.75mL）,d.add 1g sucrose per 4h（liquid fed 1mL）,after the first time add sugar interval of 4h and then add sugar,add 24h,the total concentration of sucrose medium 100g/L,added to stop,to continue to foster 48h,and measuring dry weight,program a get the highest yield of Candida Berkhout.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第7期187-188,共2页
Science and Technology of Food Industry
基金
湛江师范学院科研项目基金资助
关键词
蔗糖
假丝酵母
发酵
sucrose
Candida Berkhout
fermentation
