摘要
目的：建立HPLC法测定马鞭草药材中木犀草素、芹菜素和山柰酚的含量。方法：采用DiamonsilTMC18（250mm×4.6mm,5μm）色谱柱,以乙腈-0.05%磷酸水（40∶60）为流动相,流速1.0mL·min-1,检测波长350nm,柱温30℃。结果：木犀草素、芹菜素和山柰酚浓度分别在1.065-21.30,1.146-22.92,1.645-32.91μg.mL-1范围内与峰面积均呈良好的线性关系（r=0.9995-0.9999）;平均回收率（n=6）分别为98.1%（RSD=1.8%）,100.3%（RSD=1.5%）,97.1%（RSD=1.7%）。结论：本法操作简便、准确,具有良好的重复性,为马鞭草药材的合理应用及质量控制奠定基础。
Objective：To develop an HPLC method to simultaneously determine the contents of luteolin,apigenin and kaempferol in Verbena oficinalis L..Methods：The separation was carried out on a C18（250 mm×4.6 mm,5 μm）column with the mixture of acetonitrile-water containing 0.05% phosphoric acid（40∶60）as a mobile phase at the flow rate of 1.0 mL·min-1 and detection wavelength was set at 350 nm.The temperature of column was 30 ℃.Results：The linear relationship was good for luteolin,apigenin and kaempferol in the range of 1.065-21.30,1.146-22.92 and 1.645-32.91 μg·mL-1（r=0.9995-0.9999）.The average recoveries（n=6）were 98.1%（RSD=1.8%）,100.3%（RSD=1.5%）and 97.1%（RSD=1.7%）.Conclusion：This method is rapid,accurate and repeatable and can be used to determine the contents of luteolin,apigenin and kaempferol and control quality of Verbena oficinalis L..
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第6期995-998,共4页
Chinese Journal of Pharmaceutical Analysis
