摘要
粘液表皮样癌是涎腺恶性肿瘤中最常见的一种。本实验以六亚甲基二乙酰胺（ＨＭＢＡ）作为分化诱导剂，对涎腺粘液表皮样癌ＭＥＣ－１细胞进行了体内外诱导，并通过银染技术对对照组和ＨＭＢＡ诱导组癌细胞核内核仁组成区（ＮＯＲＳ））的数目、大小及分布特征进行了观察。结果表明：经ＨＭＢＡ诱导后的癌细胞，细胞核内嗜银颗粒的数目明显较对照组少，且直径变大，分布趋于集中。说明在ＨＭＢＡ作用下，癌细胞向成熟方向发生了一定分化，ＡｇＮＯＲＳ技术在诱导分化研究中有一定应用价值。
Mucoepidermoid carcinoma is the most common malignant tumor of salivary glands.In this paper,we used hexamethylene bisacetamide (HMBA) as a inducer to induce human mucoepidermoid carcinoma cell line MEC-1 cells to differentiate in vitro and vivo.The nucleolus organizer regions was investigated by means of the argyrophil (AgNOR) technique.The results showed that the mean number and mean diameter of AgNOR S in HMBA group were significantly different from that in control group.It suggests that HMBA can induce MEC-1 cells to differentiate toward nomal or binign cells in vitro and vivo.The argyrophil technique may play an important role in differentiation researches.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1999年第1期43-44,共2页
Cancer Research on Prevention and Treatment
关键词
涎腺肿瘤
粘液表皮样癌
HMBA
分化
AGNOR染色
Induced differentiation
nucleolar organizer region
Hexamethylene bisacetamide (HMBA)
