摘要
观察柴胡有效成分柴胡皂甙对原代培养贮脂细胞（ＦＳＣ）激活及合成细胞外基质（ＥＣＭ）的作用。结果：治疗组库普弗细胞条件培养基（ＴＫＣｎｃｍ）、治疗ＣＣｌ４损伤库普弗细胞条件培养基（ＴＫＣｔｃｍ）、ＴＫＣｎｃｍ加肝细胞条件培养基（ＰＣｎｃｍ）组ＦＳＣ内结蛋白阳性反应明显减弱，对细胞表型转化的形态学特征有一定改善，各治疗组ＦＳＣ的ＤＮＡ合成及３Ｈ－脯氨酸掺入量均呈不同程度降低，ＦＳＣ内Ⅰ型胶原含量明显减少。说明柴胡皂甙对肝细胞具有保护作用，直接抑制ＦＳＣ内ＤＮＡ的合成，还可抑制ＦＳＣ的激活，从而抑制ＦＳＣ合成ＥＣＭ的能力。
Objective: to observe the effects of Saikosaponin, an effective component of Bupleurum, on activating the primary cultured Fat Store Cells (FSC) and synthesizing extracellular matrix (ECM). Method: utilizing ultrastructure, immunocytochemistry and image pattern analysis to examine contents of collagen type 1, 3, 4 and fibronectin (FN) in FSC; using flow Cytomater to determine content of DNA and measure 3H-Proline incorporation volume so as to detect collagen protein volume in FSC. Result: positive reaction of the desmin was markedly weakened in the groups of TKCnem, TKCtem and TKCnem plus Pcnem. For the transformation of cell phenotype, the morphological character was improved. In other treatment groups on FSC, DNA synthesis and 3H-Proline incorporation volume were decreased at different levels. In all treatment groups, volume of collagen type 1 was decreased markedly, especially groups of TKCnem and TKCtem. Conclusion: Saikosaponin can protect the hepatocytes. It could inhibit the DNA synthesis and the FSC activation so to inhibit the ECM synthesis.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
1999年第1期31-34,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家教委博士点科研基金
关键词
柴胡皂甙
贮脂细胞
细胞外基质
肝纤维化
治疗
Saikosaponin
FSC
ECM
Kupffer's Cell
Immunochemistry
Liver Parenchymal Cell
