摘要
目的:探讨建立操作简便、建模周期短、成功率高的溶血性贫血(Hemolytic anemia,HA)动物模型,为开展HA的相关科学研究提供良好的实验材料。方法:随机取10只SD大鼠,采用腹腔内注射乙酰苯肼的方法制作模型,同时设立假处理组作为对照。实验处理8d后以血液分析、网织红细胞计数、骨髓象检验等实验室检查对模型进行评价。结果:第8d实验组大鼠外周血RBC、HGB、PLT、Ret%分别为(2.98±0.623)×1012个/L、(100±14.2)g/L、(320±358.8)×109个/L、90.7%±4.23%,另外间接胆红素浓度为(10.3±3.30)μmol/L、血浆游离血红蛋白浓度为(1248±282.1)mg/L,与对照组比较差异均有统计学意义;骨髓象中有核细胞增生明显活跃,以红系增生为主。结论:腹腔内注射乙酰苯肼的方法能够在较短时间内方便地制作出符合临床特征的HA动物模型,是建立HA动物模型的理想方法。
Objective：Study on establishing HA animal model,which isoperated simply and have short model building period and high successful rate,to provide favorable test materials for HA-related scientific research.Methods：10 SD rats were randomly selected to set up the model by using APH intraperitoneal injection,meanwhile rats intraperitoneally infused with saline served as controls group.After 8 days of experimental treatment,we evaluated the animal model by using blood analysis,reticular count,myelogram and other laboratory experimental results.Results：On eighth day after experimental treat,the results of RBC、HGB、PLT、Ret% in Rats＇peripheral blood are respectively（2.98±0.623）×1012/L、（100±14.2）g/L、（320±358.8）×109/L、90.7%±4.23%,the concentration of indirect bilirubin and free-haemoglobin in plasma are respectively（10.3±3.30）μmol/L、（1 248±282.1）mg/L.Compared to the control group,the difference of the results all have the statistical significance;bone marrow exhibited that karyocytes proliferate obviously,mainly the erythroid system.Conclusion：APH intraperitoneal injection can set up HA animal model shortly and conveniently,and the animal model has the clinical features.So the method of APH intraperitoneal injection is an ideal way to establish HA animal model
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2010年第5期703-705,共3页
Journal of Chongqing Medical University
基金
江西省教育厅课题(编号:JXJG-07-1-62)
关键词
溶血性贫血
大鼠模型
实验室评价
Hemolytic anemia
Rat animal model
Laboratory evaluation
