摘要
目的观察诱导后的成骨细胞在Mg-Mn-Zn表面附着与增殖情况。方法分离培养兔骨髓基质干细胞(BMSCs),将其诱导分化为成骨细胞后,以1×108/L的细胞浓度种植在Mg-Mn-Zn及对照组纯钛表面,联合培养1、3、5d后,分别用扫描电镜、激光扫描共焦显微镜观察两种金属表面的细胞形态学变化及生长情况。结果 BMSCs以球状体方式进行克隆生长,经诱导后的细胞表达成骨细胞特性,在与镁合金复合培养后,细胞发生黏附并增殖连接成片。结论诱导后的成骨细胞在Mg-Mn-Zn表面有较好的分裂增殖能力,提示镁合金有较好的骨诱导生成作用。
Objective To investigate the attachment and proliferation of osteoblasts from bone marrow stromal stem ceils （BMSCs） on the surface of Mg-Mn-Zn alloy. Methods Rabbit BMSCs were isolated by density gradient centrifugation method and amplified in the flasks, using the osteogenic inducing conditions （OGC） as the culture media. The osteogenic potential of BMSCs was investigated by means of Alizarin red staining, alkaline phosphatase （ALP） staining, collogen I immunohistochemisty technology , scanning electron microscope （SEM） and transmission electron microscope（TEM）. Besides, induced osteoblasts were seeded on the surface of Mg-Mn-Zn alloy and pure Ti by 1× 10^5/ ml. Scanning electron microscopy and laser scanning confocal microscopy were carried out to observe cell morphology and growth at 1, 3 and 5 days under co-culture condition. Results Rabbit BMSCs growed in a great number and in the form of cell spheroids and the cells outgrew in all directions firstly. These cells were then induced into osteoblasts which could adhere to the surface of Mg-Mn-Zn and connected with each other to form lamellar substances. Conclusion Osteoblasts from BMSCs have good adhesion and growth capability on the surface of Mg-Mn-Zn indicating that magnesium alloy posses good bone induction function.
出处
《解剖学报》
CAS
CSCD
北大核心
2010年第3期430-434,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30772215)
关键词
Mg-Mn-Zn合金
骨髓基质干细胞
钛
扫描电镜
激光扫描共焦显微镜
兔
Mg-Mn-Zn alloy
Bone marrow stromal stem cell
Ti
Scanning electron microscopy
Laser scanning confocal microscopy
Rabbit
