摘要
目的观察神经生长因子（ＮＧＦ）对猴基底前脑神经元的保护作用，以探讨Ａｌｚｈｅｉｍｅｒ病的治疗方法。方法将６只老年恒河猴分为２组，将其脑内的穹隆切断，阻断ＮＧＦ从大脑皮层至海马的神经通路，一组动物将能分泌人ＮＧＦ的细胞微包囊移植到同侧侧脑室内，另一组移植不能分泌ＮＧＦ的细胞。结果术后３～４周，隔区免疫组织化学检查显示，对照组隔区低亲和力神经生长因子受体（ｐ７５ＮＧＦｒ）阳性神经元较对侧减少５３％，残余细胞呈萎缩状，而实验组只较对侧减少７％～２３％；实验组的胆碱乙酰化酶免疫阳性神经元在隔区内计数比对侧减少０～３７％，而对照组减少５７％～７５％；实验组的胆碱能阳性神经元的形态正常，对照组的神经元显示萎缩；实验组在隔区内可见胆碱能神经纤维及ｐ７５ＮＧＦｒ阳性神经纤维的发芽反应，对照组无此反应。
Objective To study the preventive effects of nerve growth factor(NGF) on basal forebrain neurons to explore the therapy for Alzheimer disease.Methods Six aged Rhesus monkeys were divided into two groups(group A and group B)randomly. NGF transport from cerebral cortex were blocked in all the monkeys. Group A received homopleural intraventricular implants of polymer encapsulated cells that were modified to secrete NGF. Group B received identical implants of empty capsules.Results Three to four weeks after operation, the monkeys in the group B displayed extensive reductions in the number of p75NGFr receptor immunoreactive neurons(53%) and choline acetyltransferase immunoreactive neurons(57% to 75%)within the septum. The monkeys in group A appeared to be normal size relative to the atrophic appearance of cholinergic neurons and p75NGFr receptor immunoreactive neurons in the monkeys receiving control implants.Conclusion NGF could prevent basal forebrain neuron degeneration in aged monkeys.
出处
《中华器官移植杂志》
CAS
CSCD
1999年第1期8-9,共2页
Chinese Journal of Organ Transplantation
