摘要
为证实用日本血吸虫单克隆抗独特型抗体ＮＰ３０制备的抗体检测试剂盒考核治疗效果的价值，采用双“抗原”夹心酶联免疫吸附试验，用ＮＰ３０替代抗原，检测血吸虫病抗体。结果：该试剂盒对急性感染者的检出率为１００％，慢性轻度感染者的检出率为６１％，特异性为１００％。急性感染和慢性感染的Ｙｏｕｄｅｎ指数（ｒ）分别为１．０和０．６１，慢性感染者与经典抗体检测法ＳＥＡ／ＥＬＩＳＡ（０．６０）的ｒ无差别。用此试剂盒检测血吸虫病治疗后１ａ的血清，阴转率达１００％，明显优于经典抗体检测法（４３．６％）。结论：ＮＰ３０检测的抗血吸虫抗体属短程抗体，该试剂盒具有与循环抗原检测相同的考核疗效价值。
This investigation is designed to evaluate the value in comment on the treatment effects of the diagnostic kit made by monoclonal anti idiotypic antibody NP30 of Schistosom japonicum . The antibody of Schistosoma japonica was detected by the “antigen sandwiched” ELISA. The antigen was replaced by NP30. The results revealed that the sensitivity was 61% in the mild and chronic infection and 100% in the acute infection; and that the specificity was 100%. The Youden index of the chronic infection was 0.61. There is no difference with classical antibody detection (SEA/ELISA 0.60). The correction index of conversion to negative rate in patients cured one year later was 100%. It was superior to the classical antibody detection(43.6%). It is suggested that the antibody detected with NP30 is self short life antibody of schistosomiasis . This test kit has the value in comment on the treatment effects equal to that of circulating antigen detection.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
1999年第1期4-6,共3页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家"八五"攻关计划"血吸虫病免疫诊断"专题项目
江苏省卫生厅"日本血吸虫单克隆抗独特型抗体NP30
关键词
血吸虫病
抗独特型
抗体
短程抗体
ELISA
诊断
Schistosomiasis japonica
antibodies anti idiotypic, short life antibody
enzyme linked immun osorbent assay
