摘要
目的观察一氧化氮合酶（ＮＯＳ）活性在博莱霉素（ＢＬＭ）所致大鼠肺间质纤维化过程中的动态变化，探讨ＮＯＳ抑制剂氨基胍（ＡＧ）对大鼠肺纤维化模型的影响及其作用机制。方法观察各组动物肺脏病理组织学改变和肺匀浆中ＮＯＳ活性、丙二醛（ＭＤＡ）以及肿瘤坏死因子α（ＴＮＦα）含量的变化。结果肺匀浆中ＮＯＳ活性在气管灌注ＢＬＭ后迅速上升，第７天达高峰（与对照组相比，Ｐ＜０．０１），于第２８天基本恢复正常。ＡＧ能显著降低肺匀浆中ＮＯＳ活性，显著延缓、减轻肺泡炎。结论一氧化氮（ＮＯ）在肺间质纤维化的发生发展特别是肺泡炎阶段起着重要作用。
Objective To observe the dynamic change of the nitric oxide synthase (NOS) activity of the lung in bleomycin induced pulmonary fibrosis in rats and to investigate the effect of aminoguanidine(AG), a selective inhibitor of inducible NOS on this animal model. Methods Sixty SD rats were divided into three groups in random: control group, untreated model group, AG treated group. On experimental day (day 0), the rats were intratracheally instilled with bleomycin (5 mg/kg body weight) or sterile saline, and then treated with AG (50 mg·kg 1 ·d 1 , intraperitoneally) or saline until they were killed. On days 3, 7, 14, 28 after instillation, five rats of each group were sacrificed and the lungs were harvested for histopathological examination and determination of NOS activity, MDA and TNF. Results NOS activity in the lung homogenate was elevated quickly after bleomycin instillation and peaked on day 7, then declined. Treatment of AG remarkably ameliorated the alveolitis caused by bleomycin. The subsequently pulmonary fibrosis was also decreased, but there was no statistically significance. AG did not affect the increased level of MDA and TNF α caused by intratracheally instilled bleomycin.Conclusions Nitric oxide seems to play an important role in the development of pulmonary fibrosis, especially in the alveolitis phase.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1999年第1期51-53,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
