摘要
于1996年10月在青岛胶州湾附近海域采集中国对虾、细巧仿对虾、周氏新对虾、鹰爪虾、脊尾白虾和脊腹褐虾等6种不同科或不同属的海产虾类，用RAPD技术对其基因组DNA的多态性进行了研究。在事先优化的反应条件下，经20个随机引物扩增，共得到282条多态性片段，片段长度在230-2800bp之间。根据扩增片段的共享度计算出相对遗传距离指数，然后用UPGMA和NJ程序进行聚类分析，结果所显示的6种虾的亲缘关系与传统的分类结果基本一致，说明RAPD在海洋动物的遗传学研究中是一种有重要价值的遗传标记。
Random amplified Polymorphic DNA (RAPD) technique was applied to study thePolymorphism of genomic DNA of six marine shrimps in different families or geniues Penaeuschinensis, Parapenaeopsis tenella, Mempenaus joyneri, Trachypenaeus curvirostris, Empalaemoncarinicauda and Crangon affinis, which were collected from Jiaoztiou Bay, Qngdao in Oct 1996.Amplifications with 20 primers under predetermined optimal reartion conditions (samples were firstbeated at 94t or 5 min. and followed by 45 cycles of l min at 94t, l Ann at 36t, and 2 minat 72t, then he1d al 72t for 10 min.) gave 282 reproducible amplified fragments ranging between230 and 2 8O0hp. The amplified fragments were scored as present (1) or absent (O) for each DNAsample and an indcx of degree of band sharing (O was calculated by using Nei and Li's matehingcoefficient method. Thc value of (1 -F) was used to evaluate genetic distances between species.The phylogenetic trees were constructed with the method of bootsttap on the basis of the presenceand absence of the an1plified Polymorphic DNA fragments, and with the methods of UPGMA andNJ on the hasis of genetic distances. The results from the thrce methods of cluster analysis aresimilar in generill, and the relationships indicated by the phylogenetic trees show the differencebetween orders, families, genus and species. The results are in good overall agreement with classicaltaxonomy. it is Suggested the random amplified Polymorphic DNA (RAPD) approaches are as usefulin providing markers for marine animal genetics as they have been for other species.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1999年第1期62-67,共6页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金!3947014
国家攀登计划B资助!PD-B6-5-3
中国科学院院长基金!963054
