摘要
目的探讨阿加曲班对大鼠肢体缺血再灌注损伤对血液流变学指标的影响,为缺血再灌注损伤的防治提供新思路。方法采用大鼠肢体缺血再灌注模型,将14只Wistar大鼠随机分为2组,对照组和阿加曲班组,每组7只。2组大鼠均于再灌注2h后,酶联免疫吸附(ELISA)法测定血浆P-选择素、细胞间粘附分子-1(ICAM-1)浓度;取血检测血液流变学指标,包括血浆粘度(ηp)、全血低切还原粘度(Lηre)、全血高切还原粘度(Hηre)、红细胞压积(HCT)、红细胞聚集指数(EAI)、血沉方程K值(ESRK)、红细胞刚性指数(TK)、红细胞变形指数(EDI)。结果大鼠肢体缺血再灌注2h后,阿加曲班组血浆P-选择素浓度、血浆ICAM-1浓度显著低于对照组(P<0.01)。血液流变学指标与对照组比较,阿加曲班组ηp、Hηre、Lηre、EAI和TK明显降低(P<0.05),EDI升高(P<0.05),而对Hct、ESRK无显著影响(P<0.05)。结论阿加曲班能够降低血浆P选择素和ICAM-1浓度,进而改善大鼠肢体缺血再灌注损伤所造成的血液流变学异常。
Objective To investigate the effects of agatroban on hemorheological parameters, plasma P-selectin and plasma ICAM-1 of rats with limb ischemia/reperfusion injury in order to provide a new method for the prevention and therapy of ischemia/reperfusion injury. Methods 14 Wistar rats were randomly divided into control group and agatroban group, with 7 rats in each group. The rat model of limb ischemia/reperfusion injury was made according to the ZHANG Lianyuan＇ s ways. The plasma P-selectin and plasma ICAM-1 levels were detected by enzyme linked immunosorbent assay （ELISA） at 2h after reperfusion. The blood samples were taken and hemorheological indexes were detected, including plasma viscosity, low shear reduction viscosity, high shear reduction viscosity, hematocrit erythrocyte aggregation index, erythrocyte sedimentation rate K and erythrocyte rigi dity index. Results 2h after limb ischemia/reperfusionin rats,the plasma P-selectin and plasma ICAM-1 levels in argatroban group were significantly lower than those of control group （ P 〈 0.05 ）. As compared with those in control group, the ηP, Hηre, Lηre- EAI and Tk in argatroban group were significantly decreased （ P 〈 0.05 ）, however, EDI was obviously increased（ P 〈 0.05）, but no obvious effect on Hct, ESRK was observed. Conclusion Agatroban can reduce plasma P-selectin and plasma ICAM-1 levels, as a result, to improve hemorheological abnormality caused by limb ischemia-reperfusion injury in rats.
出处
《河北医药》
CAS
2010年第12期1529-1531,共3页
Hebei Medical Journal
