摘要
本研究采用RT-PCR克隆大豆子粒Le1基因核心保守序列515bp片段,以植物表达载体pCAMBIA1301为基本载体,构建了抑制大豆Le1基因表达的RNAi载体。通过酶切检测及测序,证明表达载体构建正确。本研究为通过RNAi技术降低大豆中凝集素含量,改良大豆品质奠定了基础。
The consensus sequence of Le1 515 bp in soybean seeds was cloned in this study by RT-PCR.A RNAi expression vector was constructed on the substructure of the plant expression vector pCAMBIA1301,that could inhibition the expression of Le1 gene.Restriction enzyme analysis and DNA sequencing showed that all the recombinant plasmids were according with our design.This study created the foundation for decreasing the activity of soybean agglutinin and improving the quality of soybeans by RNAi.
出处
《吉林农业科学》
CSCD
2010年第3期18-20,共3页
Journal of Jilin Agricultural Sciences
基金
国家自然基金(编号:30971805)
