摘要
目的通过对常染色体和X染色体遗传标记的检测,探讨全同胞姐妹关系的鉴定策略。方法提取姐妹个体的DNA,采用SinofilerTM试剂盒检验常染色体上的15个STR基因座、采用Mentype Argus X-8试剂盒和多重X染色体STR检测试剂盒检验X染色体上的17个STR基因座,同时采用TaqMan技术对11个X-SNP位点进行分型检测。结果依据常染色体STR基因座的检测结果计算全同胞指数,不排除被检个体的同胞姐妹关系;X染色体上各个STR基因座和SNP位点均检见1～2个相同的等位基因,进一步支持被检个体的同胞姐妹关系。结论对于全同胞姐妹关系的鉴定案例,除了检测常染色体STR基因座外,还可以从X染色体上选择多态性遗传标记进行检测,获得更多的遗传信息。
Objective The methods for identification of sibling sisters were explored with detection of genetic markers on autochromosome and X-chromosome.Methods Genomic DNA of the sibling sisters were extracted,and 15 STRs on autochromosome and 17 STRs on X-chromosome were genotyped by SinofilerTM kit,Mentype Argus X-8 kit and in-house kit of X-STRs,respectively.11 X-SNPs were genotyped with TaqMan technology.Results Full sibling relationship of the test samples were confirmed by calculating full sibling index of STRs in autochromosome,which were also supported by the detection of 1-2 same alleles at each locus on X-chromosome.Conclusion In identification of full sibling sister,not only STRs on autochromosome but also polymorphism genetic markers in X-chromosome can be utilized.
出处
《法医学杂志》
CAS
CSCD
2010年第3期185-187,共3页
Journal of Forensic Medicine
关键词
法医遗传学
同胞关系
X染色体
短串联重复序列
单核苷酸多态性
forensic genetics
sibling relations
X chromosome
short tandem repeat
single nucleotide polymorphism
