摘要
目的 探讨血糖波动对抵抗素的影响.方法 比较间歇性高糖和持续性高糖对人U937单核/巨噬细胞抵抗素分泌的影响;比较糖耐量正常组（NGT组,30例）、糖耐量受损组（IGT组,31例）和新诊断2型糖尿病组（T2DM组,31例）间口服葡萄糖耐量试验1 h血糖增幅（△Glu1-0）和血清抵抗索增幅（△lnRes1-0）的差异.结果 对照组、持续性高糖组和间歇性高糖组的抵抗素分泌水平分别为（73.62±5.07）ng/L、（97.78±7.00）ng/L、（212.49±28.81）ng/L,间歇性高糖组显著高于其余两组（P〈0.05）.NGT、IGT和T2DM组的△Glu1-0依次增大（P〈0.05）,分别为（2.31±2.30）mmol/L、（5.70±2.08）mmol/L、（8.41±2.63）mmol/L;其△InRea1-0分别为（0.05±0.05）μg/L、（0.25±0.04）μg/L、（0.37±0.03）μg/L,T2DM组显著大于NGT组（P〈0.05）.△lnRes1-0与△Glu1-0呈正相关（r=0.23,P=0.02）.结论 血糖波动促进单核/巨噬细胞分泌抵抗素,血糖波动幅度越大,血清抵抗素升高幅度越大,因此,对糖尿病控糖治疗,应注意减少血糖波动.
Objective To explore the effect of glucose fluctuation on resistin. Methods The phorbol-12-myristate-13-acetate（PMA）-activated and differentiated U937 cells were exposed to experimental condition for 3 days, three groups of cells were formed, each one receiving the following fresh medium every 6 hours, respectively： （1） continuous 11.1 mmol/L glucose concentration medium （Con group）, （2）continuous 22.2 mmol/L glucose concentration medium （CHG group）, （3） alternating 11.1 mmol/L glucose concentration and 22. 2 mmol/L glucose concentration medium every 6 hours （IHG group）. The supernatants of cell mediam at the last 6 hours were collected to test resistin concentration. Besides, 92 subjects were selected and classified into three groups according to the results of oral glucose tolerance test：normal glucose tolerance group （ NGT group, n =30）, impaired glucose tolerance patients （IGT group, n =31） and newly diagnosed type 2 diabetes patients （T2DM group, n =31）. Blood glucose and serum resistin levels were measured at 0 h and 1 h during oral glucose tolerance test （ OGTT） to compare the glucose fluctuation （△Glu1-0） and the change of serum resistin level （△lnRes1-0） among the three groups. Results Resistin concentration in the Con , CHG and IHG group was （73.62 ± 5.07）ng/L, （97.78 ±7.00）ng/L and（212.49 ± 28. 81 ）ng/L respectively and in IHG group it was higher as compared with the other two groups （P〈0.05）. △Glu1-0 in NGT, IGT and T2DM group was（2.31 ±2.30）mmol/L,（5.70 ±2.08）mmol/L and （8.41 ±2.63）mmol/L respectively; △Glu1-0 increased gradually in all the three groups （P〈0.05）. Serum resistin level from 0 h to 1 h in the NGT group was 6.41 （1.52-15.76） μg/L to 6. 96（ 1.52-22. 70） μg/L, in the IGT group 5.47（ 1.49-24. 09）μg/L to 9. 12（ 1.27-21.94）μg/L and in the T2DM group 5.77（ 1.11-30.10） μg/L to 9. 27（1.02-48.15）μg/L In the IGT and T2DM group serum resistin level increased from 0 h to 1 h （P〈0.05）, but no difference was observed in the NGT group （P〉0. 05）.△lnRes1-0 in these 3 groups was （0.05 ± 0.05） μg/L, （0.25 ± 0.04） μg/L and （0.37 ± 0.03 ）μg/L respectively and the change in the T2DM group was significant as compared with that in the NGT group,△lnRes1-0 was positively correlated with △Glu1-0 （r = 0.23, P = 0.02）. Conclusion Glucose fluctuation induced monocyte/macrophage to secrete resistin, greater the glucose fluctuation, greater the change of amplitude of serum resistin.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2010年第6期484-487,共4页
Chinese Journal of Internal Medicine
基金
基金项目：国家自然科学基金（30570886）
关键词
抵抗素
血糖波动
间歇性高糖
Resistin
Blood glucose fluctuation
Intermittent high glucose
