摘要
采用RAPD分子指纹技术,对86个重庆晚疫病菌菌株进行了DNA指纹扩增.结果表明:菌株间显示了DNA指纹的多态性,供试菌株扩增的条带分子量大小在500～2000bp之间.经UPGMA聚类分析,在0.80遗传相似水平下,供试菌株分为6个遗传谱系,其中谱系L1、L2、L3为优势谱系.重庆晚疫病菌的群体遗传结构较简单,菌株的遗传谱系与地理分布之间呈现一定的相关性.
DNA fingerprints of 86 Phytophthora infestans isolates collected from Chongqing were tested with RAPD-PCR. Polymorphism of DNA fingerprints appeared among the isolates. The amplified bands varied between 500 and 2 000 bp. Clustering analysis of UPGMA grouped the 86 isolates into 6 genetic lineages at 0.8 genetic similarity, with L1, L2 and L3 as the dominant lineages. The results indicated that the genetic structure of Ph. infestans populations in Chongqing was relatively simple, and the genetic lineages of isolates were related to their geographic distributions.
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第6期17-22,共6页
Journal of Southwest University(Natural Science Edition)
基金
重庆市自然科学基金资助项目(CSTC
2009BB1134)
重庆市薯类脱毒种薯快繁中心西南大学合作项目
