摘要
酿酒酵母（Ｓａｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ）Ａ００１和克鲁维酵母（Ｋｌｕｙｖｅｒｏｍｙｃｅｓｓｐ．）Ｙ０３４属间原生质体融合构建高温酵母菌株。对制备高再生活性原生质体及融合子细胞形态、生理化特征、同工酶性质、遗传稳定性和高温发酵等方面进行了研究。结果表明，融合子ＡＹ０２３和ＡＹ６８０遗传性能稳定，表达了双亲优良性状，获得了在４５℃培养条件下产酒率７４％的属间融合菌株，是目前已见文献报道的产酒率最高的高温（４５℃）酵母菌株。
Protoplast fusion between Saccharomyces cerevisiae A001 and Kluyveromyces Y034 has been studied. A001 is a high alcohol yield yeast and can ferment maltose into ethanol, but can not grow at 45℃. However, Y034 can grow above 45℃ but has a lower alcohol yield and can not ferment maltose. We obtained the fusants which were tested in several aspects such as cell morphology, physiological and biochemical feature, isozyme patterns analysis, alcohol fermentation at high temperature(45℃) and so on. The fusant AY023 with the final ethanol concentration of 7.4% has been selected, which is thermotolerant yeast with the highest alcohol yield at 45℃ until now.
出处
《微生物学报》
CAS
CSCD
北大核心
1999年第2期141-147,共7页
Acta Microbiologica Sinica
基金
农业部资助
关键词
酿酒酵母
克鲁维酵母
原生质体融合
高温菌珠
Saccharomyces cerevisiae, Kluyveromyces , Protoplast fusion, High temperature fermentation
