摘要
为获得番茄抗根结线虫杂种,以含有温度敏感型抗根结线虫Mi-1基因的高代优良自交系栽培番茄(Lycopersicon esculentum)5678161"为母本,与含有非温度敏感型抗根结线虫Mi-3基因的秘鲁番茄(Lycopersicon peruvianum)LA2823"为父本进行杂交,通过胚挽救技术获得10个胚挽救苗株系;应用序列相关扩增多态性(SRAP)分子标记技术对胚挽救苗进行亲子鉴定,证实杂种的真实性;利用Mi-1和Mi-3基因的特异引物对杂种植株进行分子检测,从杂种中检测出拥有Mi-1基因标记的后代10株,同时拥有Mi-1和Mi-3基因标记的后代1株.
To obtain hybrids resistant to root-knot nematodes of tomato,breeding line ＂5678161＂ carrying Mi-1 gene sensitive to temperature was used as the female parent and wild tomato ＂LA2823＂ carrying Mi-3 gene insensitive to temperature was used as the male parent.Ten hybrid lines were obtained by embryo rescuing method.Its realities were identified by sequence-related amplified polymorphism （SRAP） method.Ten plants with Mi-1 gene and one plant both with Mi-1 gene and Mi-3 gene were selected out by using primers specific to Mi-1 gene and Mi-3 gene.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2010年第3期255-261,共7页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省农业重大科技攻关资助项目(2009C02006-1)
浙江省面上科研农业资助项目(2007C32011)
关键词
番茄
抗根结线虫
胚挽救
序列相关扩增多态性(SRAP)
分子标记辅助选择
tomato
resistance to root-knot nematode
embryo rescue
sequence-related amplified polymorphism （SRAP）
molecular marker-assisted selection
