摘要
提出一种测定新药溴莫普林（ＢＤＰ）的微分吸附计时电位法。在Ｍｉｃｈａｅｌｉｓ（ＭＣＬ）缓冲液中（ｐＨ４．０）溴莫普林于－１．３３Ｖ左右产生一灵敏的微分吸附计时电位溶出峰．利用该峰测定ＢＤＰ的线性范围为２．０×１０－８～３．０×１０－７ｍｏｌ／Ｌ，吸附富集３ｍｉｎ检测下限为５．０×１０－９ｍｏｌ／Ｌ。探讨了影响灵敏度的主要因素，用循环伏安法，脉冲极谱等多种电化学方法研究了该体系的电化学行为和电极机理，认为所研究体系属于有吸附性的不可逆还原过程，电极反应的电子转移数为２，参与电极反应的质子数为２。用该方法测定了血清和尿样中ＢＤＰ的含量。
An electroanalytical method for the determination of brodimprim based on derivative chronopotentiometry accumulated adsorptively on the surface of a hanging mercury drop electrode has been developed.The electrochemical behaviour in MCL buffer and the mechanism of the electrode reaction have been studied by means of linear sweep voltammetry and normal pulse polarography, etc.The wave was confirmed to be irrlversible. The linear relationship between peak height and the concentration of BDP held in the range of 2.0×10 -8 ～3.0×10 -7 mol/L, with the detection limit of 5.0× 10 -9 mol/L .
出处
《分析试验室》
CAS
CSCD
1999年第1期76-78,共3页
Chinese Journal of Analysis Laboratory
关键词
溴莫普林
微分吸附
计时电位法
测定
Brodimprim
derivative adsorption chronopotentiometry
