摘要
分别应用胶束电动毛细管色谱法(MECC)、反相色谱法和串联柱HPLC法分离了左旋十八甲基炔诺酮(ING)、睾酮(T)、孕酮(P)和雌二醇(E_2)等甾体化合物MECC分离在末涂层毛细管柱上进行.当电泳缓冲溶液为含20mmlo/L的2,6-甲基-β环糊精(DM-β-CD)添加剂和50mmol/L的十二烷基硫酸钠(SDS)的10mmol/L四硼酸钠(pH9.2)时,可实现快速分离.通过与反相色谱法和串联柱HPLC法相比较可以看出,几种甾体化合物的分离机理以疏水分配作用为主,缓冲液或固定相中的环糊精同时起着空间立体选择和疏水分配作用.环糊精的空腔大小和腔内取代基的疏水性均可影响分离的选择性.
Separation of levonorgester(L), progesterone(P) , testosterone (T) and estradiol(E) was achieved by using micellar electrokinetic capillary chromatography ( MECC ) , ODS-β-CD tandem column chromatography and reversed-phased chromatography (RP-HPLC ), respectively. A column of 53 cm (to the detector) × 50 μm I.D. uncoated fused-silica capillary (totally 68 cm in length) and an ultraviolet detector with fixed wavelength at 254 nm were used in the MECC methods. The optimal run buffer in MECC was 10 mmol/L borate solution(pH 9.2) containing 20 mmol/L 2,6-dimethyl-β-cyclodextrin(DM-β-CD) modifier. The comparison of these approaches explained the hydrophobic separation mechanism with the aditional selectivity of spatial restriction and partition effect form CD additive. But the contrast of these approaches demonstrated that the interaction of the CDs' spatial restriction and partition may have some complicated effects on the peak order of the sterioids. In addition, the MECC method was proved to be more advantageous in general than the HPLC methods.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1999年第2期193-197,共5页
Chinese Journal of Analytical Chemistry
关键词
甾体化合物
环糊精
分离
测定
甾体激素
Micellar electrokinetic capillary chromatography, steroids, tandem column liquid chromatography, cyclodextrins, reversed-phase liquid chromatography
