摘要
采用分子自组装印迹技术合成了对甲氧苄氨嘧啶有高选择性的模板聚合物受体．通过Ｓｃａｔｃｈａｒｄ分析、１ＨＮＭＲ和紫外光谱研究了模板聚合物的选择结合特性．结果表明，模板聚合物主要形成一类等价结合位点，通过协同氢键作用专一地结合模板底物，其离解常数为２．２２×１０－４ｍｏｌ／Ｌ．底物的选择性结合实验表明。
A molecularly templated synthetic polymer receptor highly selective for
trimethoprim was prepared by a molecular selfassembly imprinting technique. The selective
binding characteristics of the template polymer were evaluated by Scatchard analysis, 1H NMR
study and Ultraviolet spectrometry. The results showed that a class of same binding sites,
which could specificially bind to trimethoprim by cooperative hydrogen bonding, were formed in
the polymer, and its dissociation constant was estimated to be 22210-4 mol/L. The selective
binding experiments for substrates indicated that the polymer gave much higher affinity and
selectivity for trimethoprim than for sulfanilamide and sulfapyridine. It is possiple to be a good
adsorption and binding material in the selective enrichment and determination of trace
trimethoprim in complex biosamples.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1999年第2期204-208,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
南开大学吸附分离功能高分子材料国家重点实验室资助
关键词
分子自组装
印迹技术
模板聚合物
甲氧苄氨嘧啶
Molacular selfassembly imprinting technique,
Template polymer, Trimethoprim, Substrate selectivity
