摘要
从北京两个主要牛场共采得１８７头中国荷斯坦奶牛的血样，提取基因组ＤＮＡ，通过ＰＣＲ－ＲＦＬＰ方法对Ｋａｐｐａ酪蛋白（Ｋ－ｃｎ）、Ｂｅｔａ乳球蛋白（β－ｌｇ）和Ａｌｐｈａ乳白蛋白（α－ｌａ）进行了基因型的鉴定，并结合产奶性状进行统计分析。结果表明，牛群中上述３种乳蛋白基因的基因频率分别为Ｋ－ｃｎＡ７９％，Ｋ－ｃｎＢ２１％，β－ｌｇＡ４３％，β－ｌｇＢ５７％，α－ｌａＢ１００％。Ｋ－ＣＮ和β－ｌｇ基因位点对产奶量没有显著影响（Ｐ＞０．０５），β－ｌｇ基因位点对乳脂率的影响达到了Ｐ＜０．１的显著水平。对乳蛋白基因型间的多重比较发现，β－ｌｇＡＡ型个体的乳蛋白率显著低于β－ｌｇＡＢ型和β－ｌｇＢＢ型（Ｐ＜０．０５）。
Milk protein genotypes of 187 Chinese Holstein cows were identified for Kappa casein(k-cn),Beta lactoglobulin(β-lg) and Alpha-lactalbumin(α-la) by PCR-RFLP. Gene frequencies of k-cn A and B were 79%,21%,β-Lg A and B 43% and 57% α-la B 100% .Two linear models were used to test the relationship of these loci on yield of milk and fat and protein,fat percentage and protein percentages.The only significant effect of genotype with the two models was the β-lg locus on protein percentage(P<0.1).The result of Dancun's multiple range test among different genotypes of each locus showed the protein percentage of the individuals with β-lg AA genotype was significantly lower(P<0.05)than that of those with β-lg lactoglobulin AB or BB genotype.
出处
《黄牛杂志》
1999年第1期13-16,共4页
Journal of Yellow Cattle Science
基金
国家自然科学基金
关键词
奶牛
乳蛋白基因
PCR-RFLP
产奶性状
相关分析
Dairy cattle\ Polymorphisms of milk protein gene\ PCR-RRFLP\ Dairy traits Relationship analysis
