摘要
利用EST-SSR标记对以福鼎大白茶或云南大叶茶品种为亲本选育的40个品种(系)或衍生品种(系)的亲缘关系和遗传背景进行了分析评估。28对引物在40个供试品种间共检测到99个等位位点,每对引物为2～6个,平均3.54个;遗传多态性信息量(PIC)在0.13～0.79之间,平均值为0.59;期望杂合度(He)在0.08～0.84之间,平均值为0.58;观测杂合度(Ho)在0.10～1.00之间,平均值为0.71;Shannon指数平均为1.14。按相似系数为0.55可将参试的40个品种分为四大类。研究结果发现以云南大叶茶为母本的茶树品种比福鼎大白茶为母本的品种遗传多样性更高。
40 half-sib tea cultivars related to Fuding Dabai and Yunnan Daye were used to analyze the genetic diversity and relationship based on EST-SSR markers. Totally 99 alleles were amplified using the 28 pairs of EST-SSR primers, the number of alleles per primer ranged from 2 to 6, on average of 3.54. The polymorphism information content （PIC） varied from 0.13 to 0.79, on average of 0.59. The expected heterozygosity （He） varied from 0.08 to 0.84 and the observed heterozygosity （Ho） ranged from 0.08 to 1.00, on average of 0.59 and 0.71, respectively. The Shannon index of the population was 1.14. The 40 half-sib tea cultivars were classified into 4 groups with the similarity coefficient at 0.55. The results showed that the genetic polymorphism of half-sib tea cultivars related to Fuding Dabai was more abundant than those of Yunnan Daye.
出处
《茶叶科学》
CAS
CSCD
北大核心
2010年第3期184-190,共7页
Journal of Tea Science
基金
国家"863"计划(2006AA10Z171)
"现代农业(茶叶)产业技术体系"资助
