摘要
采用牛磺胆酸钠复制大鼠急性出血坏死性胰腺炎（ＡＨＮＰ）模型，动态观察其血浆肿瘤坏死因子α（ＴＮＦα）含量、血清脂肪酶含量和胰腺病理改变等，以及抗肿瘤坏死因子α单克隆抗体（ＴＮＦαＭＣＡｂ）的治疗对它们的作用，并与假手术组比较。结果显示：假手术组大鼠的各项检测指标无异常改变，无动物死亡。ＡＨＮＰ组腹水量、血清脂肪酶及血浆ＴＮＦα水平均较假手术组显著增加，胰腺病理改变明显；经ＴＮＦαＭＣＡｂ治疗后，血浆ＴＮＦα水平未见升高，而血清脂肪酶下降，腹水积聚减少，胰腺病理改变减轻，动物死亡率显著下降。由此提示，ＴＮＦα在ＡＨＮＰ发病机理中起重要作用。
Acute hemorrhagic and necrotizing pancreatitis (AHNP) was induced by injection of sodium taurocholate into pancreatic and biliary duct of rats. TNFα MCAb was infused intravenously 15 minutes before pancreatitis was induced, and plasm TNFα level, serum lipase level and pancratic pathologic changes were tested.Results: the amount of ascites, serum lipase level and palsm TNFα level were significantly incresed and severe pancreatic pathologic changes was induced after AHNP, as compared with those in the control group .However, plasm TNFα level was not elevated after administration of TNFα MCAb, and the amount of ascites and pathologic damage to the pancreas were markely reduced. The animal fatality was reduced too. Conclusions: these suggest that TNFα play an important role in the pathogenesis of AHNP, and TNFα MCAb have a certain therapeutic effect on AHNP in rats.
出处
《中国普外基础与临床杂志》
CAS
1999年第1期15-17,共3页
Chinese Journal of Bases and Clinics In General Surgery
关键词
出血坏死性
胰腺炎
肿瘤坏死因子
单克隆抗体
Rat
Acute hemorrhagic and necrotizing pancreatitis
Tumor necrosis factor α monoclonal antibody
