摘要
利用RT-PCR和cDNA末端快速扩增法(RACE)克隆了鳜(Siniperca chuatsi)肌肉生长抑制素(myostatin,MSTN)cDNA序列,并分析了该基因的结构特征和亲缘关系。鳜MSTN cDNA序列全长2627bp,包括5′端非翻译区117bp、3′端非翻译区1376bp和开放阅读框(ORF)1134bp,共编码377个氨基酸,含22个氨基酸的信号肽。鳜MSTN具有脊椎动物MSTN的共同序列特征,含有1个蛋白酶水解位点RARR和9个保守的半胱氨酸残基。脊椎动物MSTN氨基酸序列的亲缘关系分析表明,鳜与其他鱼类聚为一支。RT-PCR分析表明,鳜MSTN在成体不同组织中的表达情况不同,其中,卵巢、肾、眼、肌肉、心、脑、皮肤和胃中有表达,肝胰脏未见表达。
The myostatin cDNA was cloned by means of RT-PCR and rapid amplification of cDNA ends （RACE）,and its structural characteristics and phylogenetic relationship were also analyzed in the Mandarin Fish,Siniperca chuatsi. The full length of mandarin fish MSTN cDNA was 2 627 bp,and it contained a 117 bp 5′-untranslated region,a 1 376 bp 3′-untranslated region and a 1 134 bp open reading frame（ ORF）,which encoded 377 amino acids with a putative signal peptide of 22 amino acids. A conservative hydrolytic site, RARR and 9 cysteine residues,the common domains of vertebrate MSTNs,were identified in mandarin fish MSTN. Mandarin fish was grouped into the same branch as other fishes in vertebrate MSTN phylogeny. RT-PCR analysis demonstrated that the mandarin fish MSTN transcript expression varied among different tissues. Its expression was detected in ovary,kidney,eye,muscle,heart,brain,skin and stomach,but not in liver.
出处
《动物学杂志》
CAS
CSCD
北大核心
2010年第3期1-10,共10页
Chinese Journal of Zoology
基金
上海市科委基础研究重点项目(No.09JC1406900)
上海市重点学科建设项目(Y1101)
