摘要
目的构建可用于白念珠菌YPD1基因敲除的质粒。方法克隆白念珠菌YPD1基因,构建YPD1载体质粒;通过酶切反应,将p5921中标记基因URA3插入载体质粒的YPD1中,形成同源重组质粒。结果成功获得YPD1载体质粒pBluescript-YPD1和敲除质粒pBluescript-YPD1-URA3。结论所获得的质粒pBluescript-YPD1-URA3可用于白念珠菌YPD1基因的同源重组敲除。
Objective To construct the plasmid for YPD1 knockout in Candida albicans.Methods The gene YPD1 of Candida albicnas was cloned in pBluescript II/SK＋.The marker gene URA3 from plasmid p5921 was inserted into the YPD1 by restriction enzyme.Results The vector plasmid pBluescript-YPD1 and knockout plasmid pBluescript-YPD1-URA3 were successfully constructed.Conclusions The plasmid pBluescript-YPD1-URA3 is helpful for homologous recombination knockout of YPD1 in Candida albicans.
出处
《中国真菌学杂志》
2010年第2期97-100,共4页
Chinese Journal of Mycology
关键词
白念珠菌
基因
质粒
Candida albicans
gene
plasmid
