摘要
目的观察小鼠腭中缝在机械扩张力作用的不同阶段CD13/APN表达的变化规律,探讨其在腭中缝机械力扩张组织改建过程中的意义。方法选用6周龄健康雄性C57BL/6小鼠90只,随机分成对照组和实验扩张组,每组各45只。用双眼簧扩弓器施加扩张力(0.56N)于实验加力组小鼠的腭中缝区。两组分别于加力后1、3、7、14、28d抽取9只小鼠通过石蜡切片免疫组织化学对CD13/APN进行组织定位,采用Western免疫印迹法半定量鉴定其表达情况,观察表达规律并与未施力的对照组比较。结果蛋白印记显示扩张组CD13/APN表达水平显著高于对照组,差异具有统计学意义。实验组7d表达水平最高,14d与28d表达呈下降,但高于对照组。扩张组1d和3d之间,14d和28d之间差异没有统计学意义。免疫组织化学染色显示CD13/APN主要定位于间充质细胞和软骨细胞膜上,阳性面积与蛋白印记结果基本对应。结论对小鼠腭中缝施加扩张力的情况下能够诱导CD13/APN的表达增加,说明CD13/AP在腭中缝扩张新骨改建过程中具有重要意义。
Objective To detect the CD13/APN in the maxillary palatal suture of mice during the mechanical force-expansion period and to discuss the significance of them in the suture remodeling after expansion.Methods Six-week-old male C57BL/6 mice were subjected to palatal suture expansion by opening loops with an initial force of 0.56 N for the periods of 1,3,5,7,14 and 28 days.The sequence of CD13/APN changes in the midpalatal suture formation of animals was detected by immunohistochemical S-ABC method and analyzed by Western-blot.Results CD13/APN could be found in the mesenchymal cell membrane and the cartilage cell membrane.The expression of CD13/APN of expansion group was markedly higher than that of the control group;the highest expression was observed in the 7-day expansion group.The amount of CD13/APN expression declined gradually from the 14-day expansion group to the 28-day expansion group;however,it was still higher than that of the control group.The expression of CD13/APN showed no distinct difference between 1-day group and 3-day group and 14-day and 28-day group.Conclusions These findings demonstrated that expansion forces across the midpalatal suture could promote the expression of CD13/APN,and it might play an important role in the bone formation and bone remodeling process.
出处
《口腔医学》
CAS
2010年第5期257-260,共4页
Stomatology
基金
国家自然科学基金资助项目(30572068
30973361)
