摘要
目的:研究含氢HTK液对供心保存过程中心肌细胞凋亡的影响。方法:SD大鼠随机分4组:对照组(保存液为HTK液)、H1组(保存液为含氢浓度约0.8mM的HTK液),H2组(含氢浓度约0.4mM的HTK液),H3组(含氢浓度约0.2mM的HTK液)。采用Langendorff离体大鼠心脏灌注法,心脏分别在各组保存液中冷保存(4℃)6h,Tunel染色测定心肌细胞凋亡率,荧光定量实时PCR检测凋亡相关基因Bcl-2和Bax的mRNA表达,分光光度法检测心肌组织中Caspase-3活性。结果:供心冷保存(4℃)6h后,H1、H2、H3组凋亡指数明显低于对照组。H1、H2、H3组的Bcl-2mRNA表达水平显著高于对照组(P<0.01或P<0.05);对照组的Bax mRNA水平明显高于H1、H2、H3组(P<0.01或P<0.05),对照组caspase-3活性明显高于H1、H2、H3组(P<0.01)。结论:含氢HTK明显抑制供心保存时大鼠心脏心肌细胞凋亡。
Objective：To investigate the effect of hydrogen HTK on cardiomyocyte apoptosis during hypothermic preservation of heart.Methods：Thirty-two SD rats were randomly divided into four groups（n=8 for each group）：Control group（the hearts were protected with HTK solution）,H1 group（Hydrogen concentration of about 0.8mM of the HTK solution）,H2 group（Hydrogen concentration of about 0.4mM of the HTK solution）,H3 group（Hydrogen concentration of about 0.2mM of the HTK solution）.The heart were harvested and mounted on the Langendorff apparatus to estimate baseline hemodynamic values.The hearts in every group underwent hypothermic（4C°） storage for 6 hours in the corresponding cardiac preservation solution.Apoptosis was manifested by TUNEL methods.Spectrophotometric method was used to detect the activity of caspase-3,and the expression levels of Bcl-2 and Bax was analyzed by real-time fluorescent quantitative FQ-PCR method.Results：In situ terminal deoxynucleotidyl transferase labeling（TUNEL）：The radio of positive cells in H1,H2,H3group was significantly lower compared with control group（P0.01or P0.05）.The capase-3 activity in control group was significantly higher than that in H1 group,H2 group,H3 group（P0.01）,and capase-3 activity in H3 group was higher than that in H1 group（P0.05）.The expression of Bcl-2 mRNA was significantly higher in H1,H2,H3 group than that in control group（P0.01or P0.05）.The expression of Bax mRNA in control group was significantly higher than that in H1,H2,H3 group.（P0.01or P0.05）.Conclusions：Hydrogen could decrease myocardial apoptosis.
出处
《现代生物医学进展》
CAS
2010年第9期1654-1657,共4页
Progress in Modern Biomedicine
关键词
氢
供心保存
缺血再灌注损伤
细胞凋亡
Hydrogen
Donor heart Preservation
Ischemia-reperfusion injury
Apoptosis
