摘要
目的探讨丁酸钠抑制人卵巢癌3AO细胞侵袭转移能力的机制。方法采用光镜和免疫组织化学染色、流式细胞术观察药物作用后细胞结构及转移抑制基因KAI1表达的变化,逆转录聚合酶链反应实验观察KAI1mRNA表达变化。结果药物作用后KAI1mRNA表达量显著高于对照组(P<0.01);KAI1基因蛋白表达较对照组明显增强(P<0.01),流式细胞术检测KAI1阳性表达率升高(P<0.05)。结论转移抑制基因KAI1的表达变化可能是丁酸钠抑制人卵巢癌细胞增殖和侵袭的机制之一。
Objective To evaluate the effects of sodium butyrate （NaB） on the metastasis of human ovarian cystadenocarcinoma cell line 3AO. Methods The morphologic changes were observed by reverse phase-contrast microscope, variation of KAI1 gene expression was estimated by immunohistochemistry, reverse-transcription polymerase chain reaction （RT-PCR） and flow eytometrY. Results The expression of KAI1 in NaB group was significantly higher than that in control group by immunohistochemistry and flow cytometry. The result of RT-PCR showed that the expression of KAIlmRNA was higher in NaB group than that in control group. Conclusion The change of KAI1 gene may be a possible mechanism that NaB can inhibit the proliferation and invasion of human ovarian cancer cell line.
出处
《河北医科大学学报》
CAS
2010年第5期531-534,545,共5页
Journal of Hebei Medical University
基金
国家自然科学基金资助项目(30940078)
河北省科学技术研究与发展计划(052761206)
河北省卫生厅科技攻关课题(04148)
关键词
卵巢肿瘤
细胞系
免疫组织化学
ovarian neoplasms
cell line
immunohistochemistry
