摘要
目的 观察急性肝功能衰竭大鼠脑组织中代谢型谷氨酸受体 1a （mGluR1a）的表达,探讨其在肝性脑病及脑水肿中的作用.方法 据随机数字表法将40只雄性SD大鼠分成2组：时照组15只,急性肝功能衰竭组25只.采用硫代乙酰胺建立急性肝功能衰竭模型,HE染色法及电镜观察脑组织细胞形态及亚细胞结构变化,免疫组化法观察脑组织mGluR1a表达,并进行图像分析.结果 通过使用硫代乙酰胺成功建立了大鼠急性肝功能衰竭脑水肿模型,与对照组相比,急性肝功能衰竭组有严重的肝性脑病表现,病理上伴随明显脑细胞水肿,脑组织免疫组化mGluR1a表达增多.结论 硫代乙酰胺能成功制成急性肝衰竭实验模型,在急性肝功能衰竭组出现明显的脑病和脑水肿表现.急性肝功能衰竭大鼠脑组织内mGluR1a表达水平升高,与肝功能衰竭大鼠脑水肿和肝性脑病的发生有关.
Objective To investigate the expression of metabotropic glutamate reccptor 1a（mGluR1a） in rat brain of acute liver failure models,and to investigate the relationship between mGluR1a and hepatic encephalopathy and cerebral edema.Methods Random number table was used to divide forty male rats into 2 groups：15 in control group and 25 in experiment group.Acute liver failure models were made using thioacetamide（TAA）,and the morphology and substructure of brain cells were observed by hematoxylin-eosin stain and transmission electron microscope.The mGluR1a expression in brain were observed by immunohistochemistry stain and quantified by computerized image analyzer.Results Acute liver failure model were built successfully.Compared with the control group,the acute liver failure rats presented severe hepatic encephalopathy,and obvious cerebral edema was observed in the brain cells.Furthermore,mGluR1 a expression was upgraded in this group.Conclusions TAA can induce acute liver failure model.The acute liver failure rats present severe hepatic encephalopathy and cerebral edema.Expression of mGluR1 a was upgraded in the brain tissue and Was associated with hepatic encephalopathy and cerebral edema.
出处
《中国综合临床》
2010年第4期380-383,共4页
Clinical Medicine of China
基金
云南省科技厅-昆明医学院联合专项资金资助项目（2007C0014R）
