摘要
[目的]观察匹维溴胺对腹泻型肠易激综合征(IBS-D)模型大鼠结肠组织血管活性肠肽(VIP)水平及其受体1(VIP-R1)表达变化,探讨匹维溴胺治疗IBS-D机制。[方法]采用应激与束缚相结合的方法复制IBS-D模型后,应用ELISA、免疫组化、逆转录聚合酶链反应(RT-PCR)检测结肠组织VIP水平及VIP-R1的表达。[结果]匹维溴胺组结肠组织中VIP及其VIP-R1 mRNA的表达均明显低于模型组和对照组(均P<0.05)。[结论]匹维溴胺治疗IBS-D模型大鼠的药理学作用与VIP水平及VIP-R1表达下调有关。
[Objective]To observe Pinaverium bromide for diarrhea-predominant irritable bowel syndrome（IBS-D）model of rat colonic vasoactive intestinal peptide（VIP）levels and its receptor 1 expression to explore the mechanism of Pinaverium bromide on IBS-D system.[Methods]IBS-D animal model was made by stress and bound method,and ELISA,immunohistochemistry,reverse transcription-polymerase chain reaction（RT-PCR）were used to detect colon tissue content of VIP and its receptor 1 expression.[Results]The VIP content in colon tissue and its receptor 1 expression was significantly lower in Pinaverium amine group than in model group.[Conclusion]The effect of Pinaverium bromide on IBS-D rats model is related to VIP and its receptor 1 expression levels.
出处
《中国中西医结合消化杂志》
CAS
2010年第2期89-92,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
国家重点基础研究发展计划(973计划)资助项目(2007CB512702)
