摘要
目的:旨在研究三叉上核(Su5)对三叉神经中脑核(Me5)神经元的活动是否发挥着重要的调节作用,从而参与对颌运动的调节。方法:本研究通过全细胞电流钳技术,刺激生后30～43d大鼠脑片上三叉上核并记录Me5神经元反应。结果:Me5神经元静息膜电位为(-53.5±0.5)mV;所有Me5神经元在超极化和去极化时分别显示为内向、外向整流;同时去极化引起神经元放电。刺激三叉上核引起4种类型的Me5神经元的反应,即逆向动作电位、GABAA、AMPA/kainate和NMDA等受体介导的反应,这些反应各占32%、36%、20%和12%。钳制电位在-60mV左右时,诱发的GABA能突触后电位为(1.08±0.45)mV,膜电位水平时;刺激引起的AMPA/Kainate受体介导的电流大小为(0.98±0.51)mV;钳制电位在-45mV左右时,NMDA受体介导的谷氨酸电流为(2.40±0.75)mV。结论:三叉上核神经元可通过突触由GABA和谷氨酸信号系统调节Me5神经元活动。
Objective：To study whether supratrigeminal nucleus（Su5）neurons play important role in modulating the activity of mesencephalic trigeminal nucleus neurons（Me5）,thus involvement in participation of jaw movement control.Methods：Whole cell current patch clamp technique was used on slice preparation to study the responses of Me5 neurons to stimulation of Su5 of rat on 30-43 days after birth.Results：Me5 neurons showed a resting membrane potential of-53.5 ± 0.5 mV.All these neurons revealed an inward rectification during hyperpolarization and outward rectification during depolarization,and they discharged when depolarized.Four types of responses to stimulation of Su5 were recorded：antidromic action potentials,GABAA receptor-,AMPA/kainate receptor-and NMDA receptor-mediated responses,showing a percentage of 32%,36%,20% and 12%,respectively.The amplitudes of postsynaptic potentials for GABAA receptor-,AMPA/kainate receptor-and NMDA receptor-mediated responses were（1.08 ± 0.45）mV（evoked at -60 mV）,（0.98 ± 0.51）mV（at resting membrane potential）and（2.40 ± 0.75）mV（at -45 mV）,respectively.Conclusion：These data suggest that Su5 neurons can modulate activity of Me5 neurons by-aminobutyric acid（GABA）and glutamate signaling system via corresponding synapses upon Me5 neurons.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2010年第1期20-25,共6页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(30470562,30600339,30470907)~~
