摘要
目的通过研究不同吸烟量对大鼠血清瘦素、脂联素、IL-6及C反应蛋白(CRP)水平的影响,探讨瘦素和脂联素等炎症因子在吸烟所致COPD全身炎症反应中的作用。方法雄性Wistar大鼠30只,随机分为大量吸烟组,小量吸烟组和对照组,每组各10只。大量吸烟组每次吸烟10支,每天吸烟2次,每周吸烟6d,共吸烟20周;小量吸烟组每次吸烟5支,吸烟时间同前。采用双抗体夹心酶联免疫吸附法(ABC-ELISA)分别检测血清瘦素、脂联素、IL-6及CRP水平。结果大量吸烟组和小量吸烟组血清瘦素和脂联素水平均较对照组降低(P均<0.05),大量吸烟组与小量吸烟组差异无统计学意义(P>0.05)。大量吸烟组和小量吸烟组血清IL-6和CRP水平均较对照组升高(P均<0.05),大量吸烟组较小量吸烟组水平高(P均<0.05)。结论吸烟可使大鼠血清瘦素与脂联素水平下降,IL-6与CRP水平升高,提示瘦素、脂联素等炎症因子可能参与吸烟所致COPD的全身炎症反应。
Objective To investigate the role of inflammatory factors like serum leptin,adiponectin,interleukin-6（IL-6）,and C-reactive protein （CRP） in the systemic inflammatory response of smoking-induced COPD.Methods Thirty male Wistar rats were randomly divided into three groups,ie.a high-dose smoking group,a low-dose smoking group,and a control group.Serum leptin,adiponectin,IL-6,and CRP levels were measured by ABC-ELISA.Results The serum leptin and adiponectin levels in both smoking groups decreased significantly compared with the control group（P0.05）,while the difference was not significant between the two smoking groups（P0.05）.The serum IL-6 and CRP levels in both smoking groups increased significantly compared with the control group（P0.05）,which were higher in the high-dose smoking group than those in the low-dose smoking group（P0.05）.Conclusions Smoking increases the serum levels of IL-6 and CRP,but reduces the serum levels of leptin and adiponectin in rats.These results suggest that leptin,adiponectin,IL-6,and CRP may be involved in the systemic inflammatory response of smoking-induced COPD.
出处
《中国呼吸与危重监护杂志》
CAS
2010年第3期298-300,共3页
Chinese Journal of Respiratory and Critical Care Medicine
关键词
慢性阻塞性肺疾病
吸烟
瘦素
脂联素
全身炎症反应
Chronic obstructive pulmonary disease
Smoking
Leptin
Adiponectin
Systemic inflammatory response
