摘要
旨在探讨溶菌酶(lysozyme,LYZ)基因与生长和产蛋性能的关系。本研究以京海黄鸡育种场培育的慢速J+和常速J-两个品系F2代育种群为试验材料,对鸡LYZ基因外显子进行了SNP检测,分析了LYZ基因与生长和产蛋性能的关系。结果,在京海黄鸡LYZ基因外显子1和2上发现了3个突变位点(G111A、T1426C、C1492T);统计分析表明,AA基因型个体12、16周龄体质量显著高于GG和GA基因型个体,开产日龄小于GG和GA基因型个体(P<0.05);TT基因型个体4、8周龄体质量和开产体质量显著低于其他基因型个体,而TN基因型个体4、8周龄体质量和开产体质量显著高于其他基因型个体(P<0.05);不同单倍型在4～16周龄体质量和开产体质量差异显著(P<0.05);群体遗传学分析表明,3个突变位点的基因型在两品系间差异显著(P≤0.05)。初步推断鸡LYZ基因可能是控制京海黄鸡生长发育的主基因或与主基因紧密连锁。
To discuss the relationship of LYZ gene with growth and laying performance traits,the second generation divergent lines of J＋ and J-female bred by Jinghai yellow chicken breeding center were used in the present study.Three SNPs（G111A,T1426C,C1492T） in exon1 and exon2 were discovered by sequencing.Multi-contrast analysis showed that the birds with genotype AA were significantly higher than those with genotype GG and GA for body weight at 12 and 16 weeks of age,and smaller than those for age of first egg（P0.05）.The birds with genotype TT were significantly lower than those with other genotypes for body weight at 4 and 8 weeks of age and body weight of first egg,while the birds with genotype TN were significantly higher（P0.05）.Analysis results indicated that the genetic effects of haplotypes on body weight of 4 to 16 weeks of age and body weight of first egg were significant.Population genetic analysis indicated that the frequency of the genotypes were significantly different in the two lines（P≤0.05）.It was concluded preliminarily that chicken LYZ gene had important effects on growth process in the chicken or it closely linked with those genes affecting growth.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2010年第5期524-530,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家肉鸡产业技术体系资助(nycytx-42G1-05)
