摘要
目的探讨阳离子卟啉TMPyP4[四-(N-甲基-4-吡啶基)]对膀胱肿瘤细胞株T24和BIU87的端粒酶活性的影响.方法 MTT法测定细胞抑制率;流式细胞仪测定细胞凋亡率;PCR-ELISA法测定肿瘤细胞的端粒酶活性;RT-PCR法测定hTERT和c-myc的mRNA表达量.结果 TMPyP4和阿霉素均表现为剂量和时间依赖的细胞抑制作用.低细胞毒浓度的TMPyP4和阿霉素作用于肿瘤细胞后均能使肿瘤细胞的凋亡率增加,端粒酶活性降低,以及hTERT和c-myc mRNA表达量降低;TMPyP4作用时间较长后,上述作用优于阿霉素.结论低细胞毒浓度的TMPyP4在药物作用时间较长后诱导细胞凋亡的作用优于阿霉素,其作用机制可能与降低hTERT活性和c-mycmRNA表达量有关.
Objective Toinvestigate the effect ofTMPyP4 on terlomerase activityof bladder carcinoma cell line T24 and BIU87.Methods The rate of cell inhibition was tested by MTT.The rate of apoptosis of the cell lines was tested by flowcytometry.The relative activity of telomerase of the cell lines was tested by PCR-ELISA.The expression of hTERT and c-myc mRNA of the cell lines was tested by RT-PCR.Results Either TMPyP4 or adriamycin showed time and dose-dependent cytotoxicity.The cell apoptosis could be induced by deuto-cytotoxic concentration of TMPyP4 and adriamycin.The telomerase activity of the cell line was decreased when the cells were treated by the deuto-cytoxic of TMPyP4 and adriamycin.The quantity of expression of hTERT and c-myc mRNA also was decreased.The TMPyP4 was better than the adriamycin when the cell lines were treated for longer time.Conclusions The effect of TMPyP4 in inducing the apoptosis of bladder neoplasma cells is better than adriamycin,and the effect of TMPyP4 may be related with inhibiting the telomerase activity.The possibile mechanism of TMPyP4 in inhibiting telomerase activity maybe related with downregulating the expression of hTERT and c-myc mRNA.
出处
《昆明医学院学报》
2010年第4期15-19,共5页
Journal of Kunming Medical College
